Competent Cells
C & E competent cells - Agrobacterium strains
Shipment on wet ice or ambient temperature.
Long time storage : Store all competent cells at -70°C.
Choosing the right preparation type for Agrobacterium strains (such as GV3101, EHA105, or AGL-1) depends on your specific plant transformation workflow:
1. Chemically Competent Cells Method:
These cells take up DNA through a heat-shock method.
Usage: They are convenient and do not require specialized equipement, though they generally yield lower transformation efficiencies compared to electroporation.
20-50 mikroliter of chemically competent cells are thawed on ice, mixed with 10 ng of plasmid DNA, heat-shocked (usually in a water bath), and then incubated in an antibiotic-free recovery medium before being plated.
2. Electrocompetent Cells Method:
These cells take up DNA using an electrical pulse (electroporation).
Usage: This is widely considered the most effective method for Agrobacterium transformation. It is highly recommended if you are introducing large plant binary vectors or creating cDNA/gDNA libraries.
Workflow: Cells are gently thawed on ice, mixed with DNA, and transferred to a chilled electroporation cuvette. They are subjected to a high-voltage pulse (e.g., field strengths of 11-18 kV/cm.
Afterward, recovery medium is added immediately to help the cells recover.
Key Handling Tips for Agrobacterium strains
Temperature Sensitivity:
Agrobacterium cells are highly sensitive to temperature changes and mechanical lysis. Always thaw them completely on wet ice and handle gently.
Mixing:
Never vortex competent cells. Always mix cell/DNA suspensions by gently tapping the tube.
Antibiotic Selection:
Ensure your growth and selection media include the appropriate resistance markers for the specific strain (e.g., Rifampicin for EHA105 or Gentamycin for GV3101).
Source : www.sansunbiologics.com / Moers (NRW) / Germany / EU
T-C1120
LBA4404 Chemically Competent Cell
€ 267,10
20*100ul
T-C1580
BJ5183 Electrocompetent cells
€ 267,10
20*50ul
T-C1610
BJ5183-AD-1 Electrocompetent cells
€ 267,10
20*50ul
T-C1620
AGL1(pSoup) Chemically Competent Cell
€ 267,10
20*50ul
T-C1640
GV3101(pSoup) Chemically Competent Cell
€ 267,10
20*100ul
T-C1650
EHA105(pSoup) Chemically Competent Cell
€ 267,10
20*100ul
T-C1630
LBA4404(pSoup) Chemically Competent Cell
€ 267,10
20*50ul
T-C3740
ClearColi BL21(DE3) Competent Cells
€ 727,90
10*100ul
T-C3600
AGL1 Chemically Competent Cell
€ 267,10
20*100ul
T-C3610
EHA105 Chemically Competent Cell
€ 267,10
20*100ul
T-C3620
GV3101 Chemically Competent cells
€ 267,10
20*100ul
T-C3630
LBA4404 Electrocompetent cells
€ 267,10
20*50ul
T-C3640
AGL1 Electrocompetent cells
€ 267,10
20*50ul
T-C3650
EHA105 Electrocompetent cell
€ 267,10
20*50ul
T-C3660
GV3101 Electrocompetent cells
€ 267,10
20*50ul
T-C3670
BL21-CodonPlus (DE3)-RIPL Competent cells
€ 267,10
20*100ul
T-C3680
S17-1 Competent cells
€ 267,10
20*100ul
T-C3690
DH10B-T1 Competent cells
€ 267,10
20*100ul
T-C3700
FT-5a Competent cell
€ 268,50
20*100ul
T-C3710
W3110(DE3)
€ 267,10
20*100ul
T-C3720
OrigamiB(DE3)pLysS Competment Cell
€ 287,10
20*100µl
T-C3730
ArcticExpress(DE3)
€ 287,10
20*100µl
T-C3750
JM108 Competent cells
€ 278,10
20*100µl
T-C3760
HT115(DE3) Competent cells
€ 245,90
20*100µl
T-C3770
GT115 Competent cells
€ 245,90
20*100µl
Haven't found the type of competent cells you were looking for? Please ask us about it!
Send your request to
eMail :info@sansunbiologics.com
Competent cells - E.coli strains
T-C1100 E.coli DH5α Competent Cells
€192,50
20*100ul
T-C1200 E.coli TOP10 Competent Cells
€192,50
20*100ul
T-C1300 E.coli JM109 Competent Cells
€192,50
20*100ul
T-C1400 E.coli BL21(DE3) Competent Cells
€192,50
20*100ul
T-C1500 BL21(DE3)pLysS Competent Cells
€192,50
20*100ul
Alle genannten Preise verstehen sich zzgl. gesetzlicher Mehrwertsteuer und Versandkosten.
Send your request to
eMail : info@sansunbiologics.com
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